A working curve is a plot of the analytical signal (the instrument or detector response) as a function of analyte concentration. These working curves are obtained by measuring the signal from a series of standards of known concentration. The working curves are then used to determine the concentration of an unknown sample, or to calibrate the linearity of an analytical instrument.
Example of a working curve
Determining the best line for calibration data is done using linear regression. Related topics:
- data handling