Science fair project... Help T.T

bomin.k · Probably a bot. Joined: 12 Feb 2006 Posts: 1

"A protein, albumin, was placed into four test tubes for each of the seven pH levels being used. Then the buffer solution for each pH level was placed into their respective tubes. After a period of ten minutes, Ninhydrin Isopropanol was placed into one of the tubes for each pH. This was repeated every ten minutes. After being centrifuged, the solutions were observed for color change and the absorbance was recorded using a spectrophotometer at 580nm. "

These are the materials needed for this science project and the procedure, but I am very confused... Actually, I get the point of this project and really want to do it, but I have no clue on how to.

when they say protien and albumin, am i suppsoed to add real food? and the buffer solution.. what shouldi use that as?? ninhydrin isopropanol. is that a liquid?

could you lay out the materials and the procedure in a way i could understand it a bit easily? Idea

RobJim · Posted: Mon Mar 13, 2006 7:03 am Post subject:

I think you're supposed to buy the protein and albumin from a scientific supply store; at least for the solutions you're going to use to make your standard curve. Then you can test real foods and things, but you need pure, standardized amounts from a store first.

vietgrl · Probably a bot. Joined: 07 Sep 2007 Posts: 1

Hi! I'm planning to do this science Fair Project too, but it seems like it requires a centifuge and spectrometer...is there any other way to do this experiment in a more simpler way? and Where do you get all the proteins from and acids from?? Thanks a lot for the help!! Please help i need help ASAP.
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