Resolution can be greatly improved using isoelectric focusing. In this technique the support gel maintains a pH gradient. As a protein migrates down the gel, it reaches a pH that is equal to its isoelectric point. At this pH the protein is netural and no longer migrates, i.e, it is focused into a sharp band on the gel.
Schematic of zone electrophoresis apparatusSpecific electrophoretic techniques
- disc electrophoresis
- capillary electrophoresis
- gel electrophoresis (SDS-PAGE)
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