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Old January 17th, 2006, 16:01
chicamex00 chicamex00 is offline
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Default Science Fair Idea Crisis

OK SO i am doing this Science Fair Expariment but i dont understand what Ninhydrin Isopropanol is? and what it does
I someone could help me i would be so happy
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Old January 17th, 2006, 16:10
chicamex00 chicamex00 is offline
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Default hi its me again

Here is the URL of the Sciece Fair Project :http://scssi.scetv.org/mims/schools/...y99/charl1.htm
This is the Avstract:
The purpose of this project was to observe how the digestion of protein varies at different pH levels. My hypothesis was that a pH level of 4 would digest the protein fastest.
A protein, albumin, was placed into four test tubes for each of the seven pH levels being used. Then, the buffer solution for each pH level was placed into their respective tubes. After a period of ten minutes, Ninhydrin Isopranol was placed one of the tubes for each pH level. This step was repeated every ten minutes. Then after being centrifuged, the solutions were observed for color change and the absorbance was recorded using a spectrophotometer at 580 nm.

As the pH level increased, the rate of digestion also increased rapidly. From a pH level of eight and up, the digestion was almost immediate. At lower pH levels, the rate of digestion was rather slow. Therefore the data given did not support the original hypothesis.

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