Help with calculations!

[RobynE]

OK, so I am trying to work out glucose concentration from a glucose oxidase method I did last week. I need to work a conversion so that concentration is read as mg/100cm3(cubed). The information I have to go on is absorbance at 550nm; it is reasonable to assume that absorbance is directly proportional to glucose concentration. To set this up proportionally (I think that's how this is done, I could be wrong) by what do I multiply/divide and how do I get these units? Is this actually really simple and I'm totally stupid and shouldn't even be studying chemistry? Do give an honest answer! Any feedback would be appreciated! :shock:

[KathChem82]

Hi!

I think you have to go through Beer's Law. A=abc. A is the absorbance, a is the molar absorptivity, b is the path length or the width of the cell used, and c is the molar concentration of the sample. There are variations though on how the Beer's Law is represented. You can also search for Beer-Lambert's Law.