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Old January 8th, 2006, 23:56
crazylilazn crazylilazn is offline
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Default I NEED MAJORR HELP PLEASE PLEASE HELP ME!!!!

Ok, i'm attempting this project which i've already confirmed, but i just found out i'm totally clueless as to what i need to do.

A protein, albumin, was placed into four test tubes for each of the seven pH levels being used. Then the buffer solution for each pH level was placed into their respective tubes. After a period of ten minutes, Ninhydrin Isopropanol was placed into one of the tubes for each pH. This was repeated every ten minutes. After being centrifuged, the solutions were observed for color change and the absorbance was recorded using a spectrophotometer at 580nm

this is what i have to do,

but HOW?

what does the Ninhydrin Isopropanol do? and why do i put it only in 1 of the 4 test tubes, and why do i need 4 test tubes for each pH level?
how do i get the buffer solution and how do i get the pH levels and WHERE can i use a spectrophotometer?!?!?!

i need HELP!!!
please answer ASAP!!!!

oh and i need to know how much of each thing to use!!!!
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