with a very high percentage yes.
if the matrix (mixture of compounds) is not too complex, the concentration of the compounds not too high (overloading the plate), its very likely.
if you are not exactly sure, a 2-D TLC is helpful. (flip the plate 90º and let it develope in an other solvent).
some compounds are colourless (UV-lamp needed)
hope thats not too confusing
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