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you need more details to find out reaction mechanism..as i understood with your writing ..you have been analyze protein and albumin mixture in different Ph's..and Ninhydrin Isopropanol is kind of reagent to use determination of deformation albumin and protein mixture in different ph at 580 nm wavelenth ..if u repeat it 4 times it may mean repeatability of analyze (increase numbering of sample and anaylze helps you to close precision in results)..but in order to esure that you should search albumin and protein behaviours in different PH then you should find out Ninhydrin Isopropanolsturucture and properties (it realy indicator or catalisor or what?) then combine all of the clues you will find right aswer easly..there must be speciifc reason for you that why youu are making analy at 580 nm..which molecule can be determine in this wavelength? this is most important question for you bc u can find what are you looking for by this way